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What does modification mean in a restriction-modification system?

Answer and explanation

Correct answer: Methylating host DNA

Restriction-modification systems protect bacterial DNA through a paired mechanism. The restriction enzyme recognizes a particular sequence and can cleave unprotected foreign DNA, whereas the modification enzyme adds methyl groups to selected bases in the same recognition sequence of the host genome. This methylation changes the recognition site sufficiently to prevent or reduce cleavage by the corresponding restriction enzyme. Therefore option C is correct. Modification does not mean burning DNA, translating it into protein, or washing an enzyme. The methyl mark is a chemical protection signal, not a change in the DNA sequence itself.

Related tags

Restriction-ModificationMethylationHost DnaRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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