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Which bond is cut by restriction enzymes in DNA?

Answer and explanation

Correct answer: Phosphodiester bond

The governing concept is the chemical structure of the DNA backbone. Nucleotides in one DNA strand are joined by phosphodiester bonds between the phosphate group of one nucleotide and the sugar of the next. A restriction endonuclease hydrolyses these backbone bonds at particular recognition sequences, producing defined DNA fragments with sticky or blunt ends. Therefore, option C is correct. Hydrogen bonds join complementary bases between the two strands, so they are not the primary bonds cut during restriction digestion. Peptide bonds belong to proteins, while glycosidic bonds connect a nitrogenous base to its sugar within one nucleotide.

Related tags

Restriction EnzymesPhosphodiester BondDna BackbonePrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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