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If too little restriction enzyme is added what can happen?

Answer and explanation

Correct answer: DNA digestion may be incomplete

If the amount of restriction enzyme is too low, it may not be sufficient to cut all recognition sites in the DNA. Some DNA molecules may remain uncut or be cut at only a few sites, producing incomplete or partial digestion. Option B is incorrect because a low enzyme amount reduces the chance of complete cutting. Exam tip: Complete digestion requires an appropriate enzyme amount, the correct buffer, and suitable incubation time.

Related tags

Restriction EnzymesDna DigestionPartial DigestionEnzyme ConcentrationBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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