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What may be observed on a gel if a restriction site is absent from the DNA?

Answer and explanation

Correct answer: An uncut DNA band

A restriction enzyme cuts DNA only when its specific recognition sequence, called a restriction site, is present and accessible. If that site is absent from the DNA molecule, the enzyme has no correct target and does not cleave the molecule. During gel electrophoresis, the DNA can therefore migrate as the original uncut molecule, producing an uncut band rather than the smaller fragments expected after digestion. Option A is correct. The gel separates nucleic-acid fragments, not free amino acids or chlorophyll. An absent restriction site also does not remove the sample tube or make the DNA disappear; it simply prevents the expected enzymatic cleavage.

Related tags

Restriction SiteUncut DnaGel ElectrophoresisRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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