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What problem can enzyme star activity cause in results?

Answer and explanation

Correct answer: Unexpected extra DNA cuts

During star activity, a restriction enzyme may cut DNA at sequences similar to, but not exactly, its normal recognition site. This produces unexpected extra DNA fragments or bands on a gel. Complete absence of DNA is not the usual effect of star activity. Exam tip: remember star activity as non-specific or relaxed-specificity DNA cleavage.

Related tags

Restriction EnzymesStar ActivityDna DigestionNon-Specific CleavageGel ElectrophoresisPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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