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A linear DNA has four restriction sites. How many fragments will form after complete digestion?

Answer and explanation

Correct answer: Five

In linear DNA, the restriction enzyme makes one cut at each restriction site. Therefore, with 4 cuts, the number of fragments is one more than the number of cuts: \(4+1=5\). Three fragments is incorrect because linear DNA does not produce fewer fragments than its cuts in complete digestion. Exam tip: For linear DNA, remember: fragments = cuts + 1.

Related tags

Restriction EnzymesLinear DnaComplete DigestionDna FragmentsRestriction SitesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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