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Which question is most exam-oriented in the restriction enzyme topic?

Answer and explanation

Correct answer: Which sequence does EcoRI recognize

A question about the recognition sequence of EcoRI is strongly exam-oriented because it tests a standard, specific fact from molecular biotechnology. EcoRI recognizes the palindromic sequence 5′-GAATTC-3′ and cleaves within this site to produce compatible sticky ends. Such enzyme–sequence matching is frequently used in direct-recall and application-based questions about restriction digestion and recombinant-DNA construction. The other choices concern everyday observations that are not central to restriction enzymes or their laboratory use. Therefore option A is the relevant examination question. Students should learn both the enzyme name and its recognition sequence rather than memorising only the general idea of DNA cutting.

Related tags

Exam OrientedEcoriGaattcRecognition SequenceRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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