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Which option gives the correct implication of protein nature of restriction enzymes?

Answer and explanation

Correct answer: Heat can reduce activity

Restriction enzymes are proteins, so their three-dimensional structure is essential for substrate recognition and catalysis. Excessive heat can disrupt weak interactions that maintain this structure, causing denaturation and a reduction or loss of enzymatic activity. The effect depends on the enzyme and exposure conditions, but the general implication in laboratory work is that temperature must be controlled. Option A is therefore correct. Restriction enzymes are not metals, and their defining function is recognition of specific DNA sequences. Like other proteins, they can become inactive under unsuitable temperature, pH, salt, or storage conditions, making options B, C, and D incorrect.

Related tags

Protein NatureHeat DenaturationEnzyme ActivityRestriction EnzymeRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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