If a mutation creates a new restriction site what may happen?

Correct answer: An additional DNA fragment may be produced

If a mutation creates a new sequence recognised by a restriction enzyme, the enzyme can cut the DNA at this additional site. This may produce an additional, usually smaller, DNA fragment and alter the band pattern on gel electrophoresis. Option B is incorrect because an extra cut can increase, not eliminate, the number of DNA fragments. Exam tip: a newly formed restriction site generally means an additional cleavage point.