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Which option gives the simple diagram idea of a blunt end?

Answer and explanation

Correct answer: Both DNA strands are cut straight at the same position

A blunt end is formed when a restriction enzyme cuts both DNA strands directly opposite each other at the same position. Therefore, no single-stranded overhang, or sticky end, is produced. In options B and C, the strands are cut at different positions, so an overhang is formed; these represent sticky ends. Exam tip: identify a blunt end by checking whether the two cuts are flush and directly opposite each other.

Related tags

Restriction EnzymesBlunt EndsDna CleavageSticky EndsBiotechnology PrinciplesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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