After restriction enzyme action which joining enzyme is next in gene cloning?restriction enzyme action के बाद gene cloning में अगला joining enzyme कौन सा है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: DNA ligaseDNA ligase
DNA ligase forms phosphodiester bonds between the ends of DNA fragments cut by restriction enzymes, thereby joining the desired gene to vector DNA. DNA polymerase synthesises a new DNA strand but does not join cut DNA fragments. Exam tip: remember restriction enzymes as “cutting” enzymes and DNA ligase as the “joining” enzyme in cloning.DNA ligase, restriction enzyme द्वारा काटे गए DNA खंडों के सिरों के बीच फॉस्फोडाइएस्टर बंध बनाकर इच्छित gene को vector DNA से जोड़ता है। DNA polymerase DNA की नई strand बनाता है, पर कटे हुए fragments को जोड़ने वाला enzyme नहीं है। परीक्षा टिप: cloning में restriction enzyme को “कटिंग” और DNA ligase को “जॉइनिंग” enzyme याद रखें।
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