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A student says restriction enzymes cut DNA anywhere. What is the correct correction?

Answer and explanation

Correct answer: They cut at specific recognition sites

The student’s statement describes random cleavage, but restriction enzymes do not normally cut DNA at arbitrary positions. Their catalytic sites recognize particular DNA recognition sequences, frequently short palindromic sequences, and cleave the backbone at a defined location within or close to that sequence. The exact result may be sticky ends or blunt ends, depending on the enzyme. Therefore option B correctly corrects the misconception. Option A is wrong because these enzymes act on DNA, while C confuses nucleotide sequences with protein sequences and D refers to a cellular structure rather than a DNA substrate.

Related tags

Recognition SiteSequence-Specific CleavageMisconceptionDna CuttingRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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