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What may happen if the recognition site is methylated?

Answer and explanation

Correct answer: Cutting may stop or decrease

Direct answer: A, cutting may stop or decrease. A restriction enzyme recognizes a particular short DNA sequence and cuts it there. Methylation means that a methyl group has been added to a DNA base. In many bacteria, methylation marks their own DNA and prevents certain restriction enzymes from cutting it. Therefore, if the recognition site is methylated, the enzyme may not bind properly or may bind but fail to cut; the result is reduced or absent cutting, not a guaranteed change in DNA length. Option A is correct because it describes this inhibition. Option B is wrong: DNA does not always become short; it may remain uncut. Option C is wrong because an enzyme cannot turn into sugar. Option D is wrong because a gel is only a separation medium and does not close. Exam cue: methylation can protect DNA from restriction digestion, but the effect depends on the enzyme and methylation site.

Related tags

MethylationRecognition SiteDna Protection

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