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What is a possible contribution of restriction enzymes in DNA fingerprinting?

Answer and explanation

Correct answer: Cutting DNA at specific recognition sequences to produce fragments of different lengths

Restriction enzymes recognise specific DNA recognition sequences and cut the DNA at those sites. Because the positions of such sites can differ among individuals, fragments of different lengths may be produced, leading to a characteristic band pattern after further analysis. Joining fragments is done by DNA ligase, whereas size-based separation is performed by gel electrophoresis. Exam tip: remember restriction enzyme for specific DNA cutting and ligase for DNA joining.

Related tags

Dna FingerprintingRestriction EnzymesDna FragmentsRecognition SequenceGel ElectrophoresisPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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