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In which scenario can blunt-end ligation be harder than sticky-end ligation?किस scenario में blunt-end ligation sticky-end ligation से कठिन हो सकती है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: When complementary overhangs are absentजब complementary overhang मौजूद न हों
Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.Blunt ends में overhang नहीं होते इसलिए base pairing सहायता नहीं मिलती। इससे ligation तुलनात्मक रूप से कठिन हो सकती है।
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