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If an enzyme is added in excess in a wrong buffer what effect may increase?

Answer and explanation

Correct answer: star activity

The governing concept is restriction-enzyme fidelity. Under unsuitable reaction conditions, such as an incorrect pH or salt concentration, excessive glycerol or enzyme concentration, prolonged incubation, or an unsuitable buffer, some restriction enzymes may lose their normal sequence specificity. They can then cut at sites that resemble, but are not identical to, the canonical recognition sequence. This relaxed, non-specific cleavage is called star activity, so option A is correct. Photosynthesis is light-driven carbohydrate production, osmosis is movement of water across a membrane, and translation is protein synthesis; none describes inaccurate restriction digestion caused by poor reaction conditions.

Related tags

Star ActivityRestriction-Enzyme FidelityBuffer ConditionsRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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