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If sticky ends of two DNA fragments are not complementary what problem will occur?

Answer and explanation

Correct answer: They will not pair easily

Direct answer: Option A, they will not pair easily. Sticky ends contain exposed single-stranded bases. Efficient pairing requires complementary base sequences: for example, A pairs with T and G pairs with C. If two fragments have non-complementary sticky ends, the exposed bases cannot form the correct hydrogen-bonded pairs, so the fragments will not align and remain together easily. DNA ligase also cannot efficiently seal a stable junction without proper alignment, although special laboratory methods may join non-compatible ends after further processing. Option A is correct. Option B, they will always join, is the opposite of the expected result. Option C, becoming chloroplasts, is biologically impossible; chloroplasts are cell organelles, not DNA fragments. Option D, becoming soil, is unrelated. Exam cue: compatible sticky ends pair; ligase seals the aligned backbone.

Related tags

Sticky EndsComplementarityDna Ligation

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