If a mutation creates a new restriction site what is possible?

Correct answer: A new extra cut and more fragments

The governing concept is that restriction enzymes cut DNA only at their specific recognition sites. If a mutation changes the nucleotide sequence so that a new recognition site appears, the corresponding enzyme can cut at this additional position. For a linear DNA molecule, one extra internal cut generally increases the number of fragments by one; for circular DNA, the new cut can alter the fragment count and sizes in the resulting pattern. Thus option A is correct. The mutation does not convert DNA into water, change an enzyme into a vitamin, or transform gel into paper. In practice, such a site change can be detected as a different restriction-fragment pattern on a gel.