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If the number of EcoRI sites increases in a DNA sample what happens to expected fragments?

Answer and explanation

Correct answer: The number of restriction fragments will increase

EcoRI cuts DNA at its specific recognition sequence. Therefore, when the number of EcoRI sites increases, complete digestion produces more cuts and hence more restriction fragments. In linear DNA, n cuts usually give n+1 fragments, whereas in circular DNA, n cuts give n fragments. The option stating that fragment number decreases is incorrect. Exam tip: Count fragments by considering both the number of restriction sites and whether the DNA is linear or circular.

Related tags

Restriction EnzymesEcoriDna FragmentsRestriction DigestionLinear DnaCircular DnaPrinciples Of BiotechnologyBiotechnology

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