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The buffer was wrong in an experiment. What may happen to restriction enzyme activity?

Answer and explanation

Correct answer: Enzyme activity may decrease or its cutting pattern may change

A restriction enzyme needs an appropriate buffer containing the correct pH, salts, and cofactors such as Mg²⁺. With a wrong buffer, enzyme activity may fall, DNA cutting may fail, or non-specific cutting may occur. Enzyme activity cannot become infinite; it depends on pH and ionic conditions. Exam tip: in restriction digestion questions, check whether the recommended buffer and its concentration are used.

Related tags

Restriction EnzymesRestriction DigestionBuffer ConditionsEnzyme ActivityDna CuttingPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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