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Which option correctly matches HaeIII with its ends?

Answer and explanation

Correct answer: HaeIII — blunt ends

HaeIII recognizes the sequence 5′-GGCC-3′ and cuts at the same position on both DNA strands. Therefore, it produces blunt ends with no single-stranded overhang. A 5′ or 3′ overhang, also called a sticky end, results from staggered cutting and is not produced by HaeIII. Exam tip: a straight cut gives blunt ends, whereas a staggered cut gives sticky ends.

Related tags

Restriction EnzymesHaeiiiBlunt EndsDna CleavageBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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