Which MCQ option gives the correct recognition sequence of EcoRI?किस MCQ में EcoRI का सही recognition sequence दिया गया है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: GAATTCGAATTC
The governing concept is restriction-enzyme specificity. EcoRI is a bacterial restriction endonuclease that recognizes the six-base-pair palindromic sequence 5′-GAATTC-3′ and cuts within this site, producing characteristic sticky ends. A palindrome reads correspondingly on the two complementary DNA strands, which is important for recognition by many restriction enzymes. Therefore option A, GAATTC, is correct. GGATCC is the familiar recognition sequence for BamHI, AAGCTT is associated with HindIII, and GGCC is associated with other restriction enzymes such as HaeIII. The alternatives are therefore plausible enzyme sequences but not EcoRI’s sequence.इस प्रश्न की मुख्य अवधारणा restriction-enzyme specificity है। EcoRI एक bacterial restriction endonuclease है, जो छह base-pair वाली palindromic sequence 5′-GAATTC-3′ को पहचानता है और इसी site के भीतर कट लगाकर sticky ends बना सकता है। Palindromic sequence का अर्थ है कि दोनों complementary DNA strands को उचित दिशा में पढ़ने पर समान pattern मिलता है; इसी कारण कई restriction enzymes ऐसी sequences को पहचानते हैं। इसलिए विकल्प A, GAATTC, सही है। GGATCC सामान्यतः BamHI, AAGCTT HindIII और GGCC अन्य enzymes जैसे HaeIII से संबंधित sequence है। इसलिए वे EcoRI का उत्तर नहीं हैं।
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