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A student says sticky ends join with complementary ends. Which principle applies?

Answer and explanation

Correct answer: Complementary base pairing

Sticky ends have single-stranded DNA overhangs. Complementary nitrogenous bases on these overhangs join through hydrogen bonds; this is complementary base pairing. It holds the DNA fragments together in the correct alignment temporarily, after which DNA ligase forms permanent phosphodiester bonds. Protein coagulation is unrelated to joining DNA ends. Exam tip: for questions on sticky ends and ligation, first identify complementary base pairing.

Related tags

Restriction EnzymesSticky EndsComplementary Base PairingDna LigationRecombinant DnaPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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