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In which condition is the chance of a desired gene inserting in reverse orientation reduced?

Answer and explanation

Correct answer: Doing directional cloning with two different restriction enzymes

Two different ends help control insert orientation. Treat this as an advantage of directional cloning.

Related tags

Directional CloningTwo EnzymesInsert OrientationRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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