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If linear DNA has no BamHI site what will happen after adding BamHI?

Answer and explanation

Correct answer: DNA will remain uncut

BamHI is a restriction endonuclease that cuts DNA only at its specific recognition sequence, 5′-GGATCC-3′. If this site is absent from linear DNA, there is no position for BamHI to cleave, so the DNA remains uncut. Linear DNA would form two fragments only if it had one BamHI site. Exam tip: for linear DNA, the number of fragments is usually one more than the number of cuts.

Related tags

Restriction EnzymesBamhiRecognition SequenceLinear DnaDna CleavagePrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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