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A linear DNA has three restriction sites. How many fragments will form after complete digestion?

Answer and explanation

Correct answer: Four

In linear DNA, the restriction enzyme makes one cut at each restriction site. Therefore, the number of fragments equals the number of cuts plus one. Three cuts produce four fragments. The option three is incorrect because it equals the number of cuts, not the number of fragments. Exam tip: For linear DNA, remember: fragments = restriction sites + 1.

Related tags

Restriction EnzymesLinear DnaRestriction SitesDna FragmentsComplete DigestionPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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