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A plasmid is cut at a single restriction site. What will be the shape of the circular plasmid now?

Answer and explanation

Correct answer: Linear

When both DNA strands of a circular plasmid are cut at one restriction site, the circle opens to form one linear DNA molecule. A nicked circular plasmid results when only one strand is broken, whereas a restriction enzyme cut normally cleaves both strands. Exam tip: one complete cut in circular DNA gives one linear fragment; two cuts give two linear fragments.

Related tags

Plasmid DnaRestriction EnzymesDna DigestionCircular DnaLinear DnaPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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