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What does complete restriction digestion mean?

Answer and explanation

Correct answer: Cutting at all available recognition sites

Complete restriction digestion means that the selected restriction enzyme has cleaved every accessible copy of its recognition sequence in the DNA sample under the stated reaction conditions. Consequently, the DNA produces the expected set of fragments for that restriction map, without a substantial population of molecules remaining uncut or only partly cut. Option A expresses this governing principle. Complete digestion does not mean that DNA is converted into protein, that the electrophoresis gel disappears, or that a cell changes colour. Those alternatives confuse DNA cleavage with translation, laboratory material behaviour or cell physiology. Proper enzyme amount, buffer, temperature and incubation time help achieve complete digestion.

Related tags

Complete DigestionRecognition SitesRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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