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PstI is associated with which recognition sequence?

Answer and explanation

Correct answer: CTGCAG

Restriction enzymes recognize particular short DNA sequences and cut DNA at or near those sites. PstI is a restriction endonuclease whose recognition sequence is 5′-CTGCAG-3′. It produces compatible sticky ends when it cleaves the DNA at its specific site. Therefore, option A, CTGCAG, is correct. AAGCTT is recognized by HindIII, GAATTC is associated with EcoRI, and GGCC is the central recognition sequence associated with HaeIII in standard examples. The key principle is that each restriction enzyme has a characteristic recognition sequence, so the enzyme name must be matched with the correct DNA base order.

Related tags

PstiRestriction-EnzymesDna-Recognition-SequenceBiotechnologyRestriction EnzymesPrinciples Of BiotechnologyClass 11 Mcq

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