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Which enzyme is famous for producing blunt ends at the GGCC sequence?

Answer and explanation

Correct answer: HaeIII

HaeIII is a restriction endonuclease whose recognition sequence is 5′-GGCC-3′, with the complementary strand also carrying GGCC in the corresponding orientation. It cuts symmetrically in the recognition site, so the two DNA strands end at the same position and no single-stranded overhang is left; the result is a blunt end. Thus option A is correct. EcoRI, BamHI and PstI are also restriction enzymes, but they are commonly associated with staggered cleavage and sticky ends rather than this specific GGCC blunt-end example. The question tests recognition of a named enzyme and its cleavage pattern.

Related tags

HaeiiiGgccBlunt EndsRestriction EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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