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What makes restriction enzymes precise tools in cloning?

Answer and explanation

Correct answer: Fixed recognition site

The governing concept is sequence-specific recognition. A restriction enzyme does not cut DNA randomly; its active site recognises a particular short nucleotide sequence called the recognition site, often a palindromic sequence, and cleaves at a defined position within or near that site. Because the same sequence can be located and cut reproducibly, researchers can excise a selected gene or open a vector in a controlled manner. Therefore option A, fixed recognition site, is correct. Loud sound, sweet taste, and round shape are physical descriptions unrelated to the catalytic specificity of an enzyme. This sequence-directed action is what makes restriction enzymes valuable tools in cloning.

Related tags

Recognition SiteRestriction EnzymesCloningPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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