Which statement gives the correct difference between restriction enzyme and DNA ligase?

Correct answer: Restriction enzyme cuts and ligase joins

The governing concept is the division of enzymatic roles in recombinant-DNA technology. Restriction enzymes recognise specific DNA sequences and cleave the phosphodiester backbone, thereby producing defined DNA fragments with blunt or sticky ends. DNA ligase performs the opposite construction step: after compatible fragments are aligned, it catalyses phosphodiester-bond formation and seals the backbone. Thus option A accurately states that the restriction enzyme cuts and ligase joins. Option B incorrectly assigns both enzymes a lipid-digesting function. Option C reverses the relevant roles and adds an incorrect staining claim, while option D describes neither enzyme. The distinction is essential for understanding cloning workflows.