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Subjects
Class 11BiotechnologyPrinciples of BiotechnologyRestriction EnzymesEasy
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Restriction enzymes cut bonds in which backbone of DNA?

Answer and explanation

Correct answer: Sugar-phosphate backbone

Restriction enzymes recognize specific DNA sequences and cleave phosphodiester bonds in the sugar-phosphate backbone. Hydrogen bonds between nitrogenous bases hold the two strands together, but the actual permanent cut is made in the phosphodiester linkage between sugar and phosphate. Exam tip: associate the DNA backbone (sugar + phosphate) with phosphodiester bonds.

Related tags

Restriction EnzymesDna BackbonePhosphodiester BondBiotechnologyDna CleavagePrinciples Of BiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1Sticky ends are produced by what?
  2. 2What is the advantage of cutting both gene and plasmid with the same restriction enzyme?
  3. 3What are restriction enzymes mainly used for?
  4. 4Where are restriction enzymes mostly found naturally?
  5. 5Restriction enzymes help bacteria protect themselves from what?
  6. 6What do restriction enzymes recognize in DNA?
  7. 7Why are Type II restriction enzymes more useful in biotechnology?
  8. 8EcoRI is a restriction enzyme obtained from which bacterium?
  9. 9What type of ends does EcoRI usually produce?
  10. 10Why are sticky ends useful?
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