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In which condition can two DNA fragments join more easily?

Answer and explanation

Correct answer: When they have complementary sticky ends

DNA fragments with complementary sticky ends can anneal because their single-stranded overhangs form hydrogen bonds by complementary base pairing. DNA ligase then forms phosphodiester bonds in the sugar-phosphate backbone to make the joining permanent. Non-complementary ends cannot readily undergo this initial base pairing. Exam tip: DNA fragments cut by the same restriction enzyme often have complementary sticky ends.

Related tags

Restriction EnzymesSticky EndsComplementary Base PairingDna LigaseRecombinant DnaPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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