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What type of ends does EcoRI usually produce?

Answer and explanation

Correct answer: 5′ single-stranded overhangs (sticky ends)

EcoRI is a restriction enzyme that cuts the recognition sequence 5′-GAATTC-3′ in a staggered manner. This produces sticky ends with 5′ single-stranded overhangs, which can base-pair with complementary DNA ends. A 3′ overhang can also be sticky, but EcoRI specifically produces a 5′ overhang. Exam tip: remember EcoRI with the GAATTC recognition site and the 5′-AATT overhang.

Related tags

EcoriRestriction EnzymesSticky EndsDna OverhangsRecombinant DnaPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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