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Why are Type II restriction enzymes more useful in biotechnology?

Answer and explanation

Correct answer: They cut DNA near recognition sites

Type II restriction enzymes are useful because they recognize particular short DNA sequences and cut at defined positions within or close to those sites. Their predictable action produces reproducible DNA fragments. Scientists can therefore cut a vector and a desired DNA insert in a planned way and join compatible ends during cloning. This control is the important advantage; random cutting would make the result difficult to predict.

The correct statement is that these enzymes cut DNA near recognition sites, so option D follows. They do not cut proteins, manufacture RNA, or act without recognition. A small terminology point is that many Type II enzymes cut within their recognition sequence, while some related enzymes cut nearby. Thus “near recognition sites” is the best available choice and expresses their site-specific behavior used in recombinant DNA work.

Related tags

Type Ii EnzymesDna CloningRestriction Enzymes

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