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Why is DNA ligase considered necessary in genetic engineering?

Answer and explanation

Correct answer: Because it joins DNA fragments

DNA ligase permanently joins two DNA fragments by forming phosphodiester bonds between them. In genetic engineering, it is used to insert a desired gene into vector DNA. Cutting DNA at specific sites is the role of restriction enzymes, not ligase. Exam tip: remember ligase as the “molecular glue” that joins DNA fragments.

Related tags

Dna LigaseGenetic EngineeringRecombinant DnaRestriction EnzymesVector DnaLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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