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What is the function of DNA ligase enzyme?

Answer and explanation

Correct answer: To join DNA fragments by forming phosphodiester bonds between them

DNA ligase joins cut or nicked DNA ends by forming phosphodiester bonds. Therefore, it is used in recombinant DNA technology to join an inserted DNA fragment with a vector. The function in option B belongs to a restriction enzyme, which cuts DNA at specific recognition sequences. Exam tip: remember ligase as the “molecular glue” of DNA.

Related tags

Dna LigaseGenetic EngineeringRecombinant DnaPhosphodiester BondRestriction EnzymeBiotechnology EnzymesLigase EnzymesPrinciples Of Biotechnology

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