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What is the best reason for calling ligase molecular glue?

Answer and explanation

Correct answer: It joins DNA pieces by forming covalent phosphodiester bonds

The expression molecular glue is an analogy for the joining action of DNA ligase. When two DNA ends are correctly aligned, ligase catalyses formation of covalent phosphodiester bonds in the sugar-phosphate backbone, converting separate fragments into one continuous DNA molecule. Thus option A gives the best reason. This joining is not merely a weak physical association; it is a chemically stable bond-forming reaction. Option B describes restriction enzymes or other nucleases, while C concerns staining methods used for visualisation. Option D is opposite to ligase function because ligase preserves and assembles DNA rather than removing it.

Related tags

Molecular GlueCovalent BondDna LigaseLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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