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Which option gives a wrong role of ligase?

Answer and explanation

Correct answer: Cutting DNA at a restriction site

DNA ligase is a sealing and joining enzyme. It can close nicks in a DNA strand, join Okazaki fragments during lagging-strand synthesis, and connect a vector with a foreign DNA insert during recombinant-DNA formation. These functions involve creating phosphodiester bonds in the DNA backbone. Therefore option D is the wrong role. Cutting DNA at a restriction site is carried out by a restriction endonuclease, which recognises a specific sequence and cleaves the DNA. The distinction is important: restriction enzymes cut, whereas ligase joins or seals the resulting DNA ends.

Related tags

Ligase FunctionOkazaki FragmentsRestriction EnzymeLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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