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Which option correctly relates ligase and nick?

Answer and explanation

Correct answer: Ligase seals a nick by joining the sugar-phosphate backbone of DNA

A nick is a small break in the sugar-phosphate backbone of one DNA strand. DNA ligase seals this break by forming a phosphodiester bond, so option A is correct. DNA polymerase adds nucleotides, whereas restriction enzymes cut DNA at specific sequences. Exam tip: remember ligase as the enzyme that ‘joins’ broken DNA ends or seals nicks.

Related tags

Dna LigaseDna NickPhosphodiester BondRecombinant DnaBiotechnology EnzymesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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