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How does ligase maintain DNA continuity?

Answer and explanation

Correct answer: By sealing a nick in the DNA backbone

DNA ligase joins a nick in the sugar-phosphate backbone of DNA by restoring the phosphodiester bond, making the strand continuous again. Cutting DNA is the role of nucleases, not ligase. Exam tip: remember ligase as the enzyme that “joins” DNA fragments or seals nicks.

Related tags

Dna LigaseDna ContinuityPhosphodiester BondDna BackboneNick SealingBiotechnology EnzymesLigase EnzymesPrinciples Of Biotechnology

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