Update

Muft Shiksha™ एक 100% Free Education Portal है 🇮🇳, जिसका उद्देश्य Class 9–12 के हर विद्यार्थी तक High-Quality Education को पूरी तरह मुफ्त पहुँचाना है। 🇮🇳 हम मानते हैं कि अच्छी शिक्षा किसी student की आर्थिक स्थिति पर निर्भर नहीं होनी चाहिए। 🇮🇳 हर विद्यार्थी को वही Quality Study Material, MCQs, Quizzes, Exam Preparation, Concept-Based Learning और Bilingual Support मिलना चाहिए, जो आमतौर पर महंगी Coaching या Premium Platforms में मिलता है। Muft Shiksha™ 🇮🇳 इसी सोच के साथ बनाया गया है

Subjects
0 reads0 ratings0 helpful

If a ligase mixture lacks ATP and the enzyme is T4 ligase what may happen?

Answer and explanation

Correct answer: Ligation may be greatly reduced or fail

T4 DNA ligase requires ATP to form phosphodiester bonds between DNA ends. Without ATP, enzyme activation and the ligation reaction can stop, so ligation may be greatly reduced or fail. Cutting DNA is the role of a restriction endonuclease, not a ligase. Exam tip: Remember that T4 DNA ligase is ATP-dependent.

Related tags

T4 Dna LigaseAtp DependenceDna LigationPhosphodiester BondRestriction EnzymesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

Was this question useful?

No ratings yetWrite a review / Rate this question

Student Reviews

No published reviews yet.

Add Muft Shiksha to your Home Screen

In Safari, tap Share, then Add to Home Screen.