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If vector is dephosphorylated what may be important for insert ligation?अगर vector dephosphorylated है तो insert ligation के लिए क्या महत्वपूर्ण हो सकता है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Insert has proper phosphate endsinsert के पास सही phosphate ends हों
Dephosphorylated vector reduces self-ligation but proper insert ends help ligation. Chemical ends must be considered.Dephosphorylated vector self-ligation घटाता है लेकिन proper insert ends ligation में मदद करते हैं। Chemical ends का ध्यान जरूरी है।
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