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E coli DNA ligase can be distinguished by which cofactor?

Answer and explanation

Correct answer: NAD⁺

E. coli DNA ligase uses NAD⁺ as a cofactor to form phosphodiester bonds between broken DNA ends. In contrast, many viral DNA ligases, such as T4 DNA ligase, are ATP-dependent. FAD and biotin are not cofactors for this ligation reaction. Exam tip: remember bacterial DNA ligase as generally NAD⁺-dependent and T4 DNA ligase as ATP-dependent.

Related tags

Dna LigaseNad CofactorE ColiBiotechnologyRestriction EnzymesDna ReplicationLigase EnzymesPrinciples Of Biotechnology

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