If ligase is not added to a cloning mixture what will be missing?अगर cloning mixture में ligase नहीं डाला गया तो क्या कमी रहेगी?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: permanent DNA joiningpermanent DNA joining
Without ligase permanent phosphodiester joining will not occur. Sticky ends can only pair temporarily.Ligase न होने पर permanent phosphodiester joining नहीं होगी। Sticky ends केवल temporary pair कर सकते हैं।
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