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Which option is the best revision point for the ligase topic?

Answer and explanation

Correct answer: Joins DNA by forming phosphodiester bonds

DNA ligase is fundamentally a joining enzyme. It brings adjacent DNA ends together and catalyses formation of a phosphodiester bond between the 3′-hydroxyl group and the 5′-phosphate group of the DNA backbone. This action seals a nick or joins compatible DNA fragments, which is essential during recombinant-DNA construction and DNA repair. Therefore option A is correct. Restriction enzymes, represented by option B, cut DNA at particular recognition sequences rather than joining it. Options C and D describe unrelated processes involving carbohydrate digestion and plant pigment formation, so they do not identify ligase activity.

Related tags

Dna LigasePhosphodiester BondDna JoiningLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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