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Why can self-ligation decrease if vector ends are dephosphorylated?अगर vector ends dephosphorylated हैं तो self-ligation क्यों घट सकती है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Removal of 5 prime phosphate makes vector ends less able to join themselves5 prime phosphate हटने से vector अपने ends आसानी से नहीं जोड़ पाएगा
Removing 5 prime phosphate can reduce vector self-ligation. This is a strategy to reduce unwanted background in cloning.5 prime phosphate हटने पर vector self-ligation कम हो सकती है। यह cloning में unwanted background घटाने की strategy है।
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