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Which enzyme can be considered molecular glue in cloning?

Answer and explanation

Correct answer: DNA ligase

DNA ligase joins cut DNA fragments by forming phosphodiester bonds between their ends. Therefore, it is called the molecular glue in recombinant DNA technology and cloning. A restriction endonuclease cuts DNA at specific sites, whereas ligase seals the cut ends together. Exam tip: restriction enzymes cut; ligases join.

Related tags

Dna LigaseMolecular CloningRecombinant DnaRestriction EnzymesPhosphodiester BondLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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