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Which option is correct for ligase storage practice?

Answer and explanation

Correct answer: Keep it under cold conditions

The governing concept is maintaining the stability and catalytic activity of a protein enzyme during storage. Ligase can lose activity if exposed to excessive heat, repeated warming, unsuitable pH, or other damaging conditions. Cold storage, using the temperature and buffer specified by the manufacturer, slows denaturation and degradation and therefore helps preserve functional enzyme molecules. Option A is correct. Open sunlight may heat the sample and can expose it to damaging radiation; boiling is expected to denature most proteins; and dry soil is neither sterile nor a controlled storage medium. In practice, aliquoting and avoiding repeated freeze-thaw cycles may further protect activity, but the essential exam answer is cold storage.

Related tags

Ligase StorageCold ConditionEnzyme StabilityFreeze-ThawLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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